Fig. 4

Phytohormone-related miRNAs involved in seed maturation and seed germination processes. a: Overview of the miRNA-mediated regulations in phytohormone crosstalk through KEGG pathway analysis; b: sRNA sequencing data showing the expression of miRNA during seed development and germination. The expression levels are visualized by BAR HeatMapper. Numbers beneath the heat map indicate the relative expression intensities, and the higher expression intensities are indicated by more reddish colors; c: Northern blot analysis of miRNA levels in embryos during seed development. 30 μg and 50 μg (for miR393) total RNA was loaded, and rRNA was stained by EtBr as loading control. E, embryo; d: Northern blot analysis of miRNA levels during early seedling growth. Total RNA was isolated from tissues in different development stages. L, leaf; R, root; S, stem; 1 L, first leaf; 2 L, second leaf