Fig. 1
Development and molecular characterization of transgenic sweetpotato plants overexpressing AtP3B. a Schematic diagram of the vector construct containing AtP3B under the control of the CaMV 35S promoter. b Genomic DNA PCR analysis using the 35Spro::AtP3B primer set. PC, positive control. c qRT-PCR analysis of transgenic sweetpotato plants overexpressing AtP3B. Three independent transgenic lines (#1, #30, and #32) were selected for further characterization. d Southern blot analysis of OP plants; the integration and gene copy number of the construct in OP plants were confirmed using a 32P–labeled probe designed based on the AtP3B cDNA fragment