Fig. 2
From: Catalytic and functional aspects of different isozymes of glycolate oxidase in rice
Expression of GLO isoforms in yeast. a Western blotting analysis of each GLO isoform expressed in yeast. CK represents the crude enzyme extracted from yeast cells transformed with pYES3/CT vector. Nhis-GLO1, Nhis-GLO3, Nhis-GLO4 and Nhis-GLO5 represent the crude enzyme extracted from yeast cells transformed with pYES3-Nhis-GLO1, pYES3-Nhis-GLO3, pYES3-Nhis-GLO4, pYES3-Nhis-GLO5 respectively. Nhis-GLO1 + 4 represent the crude enzyme extracted from yeast cells co-transformed with pYES3-Nhis-GLO1 and pYES2-Nhis-GLO4. b Substrate specificity of each GLO isoform. Values are means ± SD (n = 3). c The purity and subunit molecular weights of the GLO isoforms purified from yeast. GLO isoforms were purified from yeast cells by immobilized metal affinity chromatography, and the molecular weights of the subunits were determined by uniform SDS-PAGE (12.5%). The results are representative of three independent experiments