Fig. 5

Effect of VOCs produced by Rs on the growth of PGPR strain SYST2. Ten μl of (18–24 h) Rs culture (1 × 10⁷ CFU ml⁻¹) grown in CPG broth was dropped in one partition of I-plate while 10 μl of the liquid overnight-grown culture of SYST2 (1 × 10⁸ CFU ml⁻¹) was dropped in the other partition containing modified minimal salt medium (MS). The plates were double sealed and incubated at 28 °C for three days. Only pathogen and only SYST2 were inoculated on control plates. The diameter of Rs and SYST2 were measured in mm. Only SYST2 (a1), SYST2 with SYST2 (a2), SYST2 with Rs (a3), only Rs (a4) and Rs with Rs (a5). Effect of VOCs produced by pathogenic strain Rs on SYST2 growth (b1) and SYST2-VOCs on Rs growth was observed (b2) and percentage growth inhibition (b3) was recorded. Similarly, the effect of specific VOCs produced by Rs was evaluated (c). Error bars indicate the standard deviation from the mean. Each experiment was performed with five replicates, and the experiment was repeated three times