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Fig. 2 | BMC Plant Biology

Fig. 2

From: GaMYB85, an R2R3 MYB gene, in transgenic Arabidopsis plays an important role in drought tolerance

Fig. 2

Gel and qRT-PCR analysis of GaMYB85 transgenic plants a The confirmation of GaMYB85 gene CDS (771 bp) integration in T0 generated overexpressed Arabidopsis plants. The DNA of WT (Col-0) was used as negative control (lane 1) and 35S:GaMYB85 DNA was used in (lanes 1–10, 11–16). M; DNA marker III, 1 kb. b Relative expression of GaMYB85 gene in T2 transgenic lines by qRT-PCR having segregating ratio of 3:1 on the selective medium, three cDNA preparations were used and error bars represented with SD value. AtUBQ10 gene (Accession no: AT4G05320) was used as an internal standard in qRT-PCR

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