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Fig. 5 | BMC Plant Biology

Fig. 5

From: Transcriptomic changes reveal gene networks responding to the overexpression of a blueberry DWARF AND DELAYED FLOWERING 1 gene in transgenic blueberry plants

Fig. 5

Differentially expressed genes (in comparison to non-transgenic ‘Legacy’) in dormant bud tissues of ‘Legacy-DDF1-OX’ plants. a Major phytohormone gene contigs, i.e., gibberellin (GA), abscisic acid (ABA), ARR related genes of cytokinin, and indole acetic acid (IAA). b qRT-PCR analysis of representative transcripts. Eukaryotic translation initiation factor 3 subunit H is the internal control. Relative expression (fold-change) in Legacy-VcDDF1-OX was calculated by 2-∆∆Ct, ∆∆Ct = (CtGOI – Ctnom)Legacy-VcDDF1-OX – (CtGOI – Ctnom)Legacy. Average fold-changes ± standard error of three biological replicates for each of ‘Legacy-VcDDF1-OX’ and ‘Legacy’ plants were plotted. Significant average fold-change determined using a Student’s t-test is denoted. An asterisk (*) indicates p < 0.05; double asterisks (**) indicate p < 0.01; and triple asterisks (***) indicate p < 0.001

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BMC Plant Biology

ISSN: 1471-2229

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