Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

Fig. 3

Analysis of the endogenous ABA and ABA- glucose ester (GE) levels following sinapic acid pretreatment of seeds in Arabidopsis. a qRT-PCR analyses of ABA UDP-glucosyltransferase genes after pretreatment of seeds with sinapic acid or DMSO (mock-treated control). The seeds were incubated with 0.5 mM sinapic acid or DMSO for 36 h at 4 °C in the dark and then germinated on MS agar medium for 1 d. Total RNAs were isolated from the treated and mock-treated seeds; Actin2 primers were used in PCR as an internal control. b Analysis of endogenous ABA and ABA-GE levels using HPLC-mass spectrometry. Seeds were immersed in 0.5 mM sinapic acid or DMSO (mock) for 36 h at 4 °C in the dark and then transferred to MS medium for 1 d of germination. Data are based on three independent replicates (± SD). c Analysis of endogenous ABA and ABA-GE levels in fah1–1 Arabidopsis plants. Seeds were immersed in water for 36 h at 4 °C in the dark and then transferred to MS medium for 1 d of germination. Data are based on three independent replicates (± SD). Asterisks indicate significant changes compared with the control (P < 0.05) calculated using Student’s t-test

Back to article page