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Table 2 Glycosyl linkage compositions of cell walls and fractions from collenchyma strands from middle petiole segments

From: Polysaccharide compositions of collenchyma cell walls from celery (Apium graveolens L.) petioles

Monosaccharides

Deduced glycosyl linkagea

Fractions

 

HEPES

Cell wall

CDTA

Na2CO3

1 M KOH

4 M KOH

Residue

Deduced polymer

Rhamnose

terminalp

1.06 (1.13)

0.09

0.31

0.69

0.27

0.21

0.04

RG-II/AG-II

2p

1.68 (1.19)

1.14

2.48

5.90

2.28

1.91

0.38

RG-I backbone

2,4p

0.90 (0.82)

0.36

1.04

1.96

0.83

0.71

0.06

RG-I backbone

Fucose

terminalp

0.29 (0.82)

0.33

0.22

0.40

1.38

3.63

-

RG-II/XGs

Arabinose

terminalf

6.47 (7.28)

0.94

2.14

4.16

1.85

1.21

0.35

RG-I/RG-II/AG-II/HXs

terminalp

0.45 (0.42)

-

-

-

-

-

-

AG-II

3f

0.40 (0.66)

-

-

-

-

-

-

RG-I

5f

4.51 (4.46)

1.53

5.05

6.64

2.69

1.72

0.44

RG-I

3,5f

0.91 (1.01)

0.60

1.80

3.06

1.26

0.78

-

RG-I

Xylose

terminalp

2.37 (3.62)

2.26

0.46

0.51

5.91

12.4

0.25

HXs/XGs/other

2p

-

0.81

-

-

3.92

4.54

0.05

XGs

4p

34.7 (31.9)

2.81

0.47

1.12

26.5

3.73

0.17

HXs

2,4p

5.36 (5.04)

0.39

-

-

4.04

0.62

-

HXs

2,3,4p

0.84 (0.66)

0.31

-

-

0.31

0.32

-

HXs

Mannose

4p

1.24 (0.97)

1.24

0.42

0.86

2.90

3.88

0.67

HMs

4,6p

- (−)

0.78

-

-

1.33

1.86

0.06

HMs

Galactose

terminalp

1.69 (1.84)

0.88

0.76

1.99

3.02

4.93

0.36

RG-I/RG-II/AG-II/XGs/HMs

2p

0.28 (0.94)

0.50

-

-

2.71

5.26

0.06

XGs/HMs

3p

0.57 (0.81)

-

-

-

-

-

-

AG-II

4p

1.14 (1.19)

1.39

1.74

3.35

1.59

1.77

0.83

RG-I

6p

3.19 (2.58)

0.47

1.18

2.35

1.00

1.01

0.35

AG-II

2,4p

- (0.30)

-

0.82

-

0.21

0.32

-

RG-II

3,6p

5.46 (5.81)

-

-

-

-

-

-

AG-II

3,4,6p

0.40 (0.55)

-

-

-

-

-

-

AG-II

Glucose

terminalp

1.37 (1.14)

0.43

0.29

0.73

-

0.50

0.30

Other

4p

4.63 (4.45)

54.1

1.89

3.89

9.23

18.2

85.5

XGs/HMs/Cellulose/other

2,4p

- (−)

0.77

-

-

-

-

0.13

Other

3,4p

- (−)

0.99

-

-

-

-

0.33

Other

4,6p

1.76 (3.91)

4.16

-

-

7.25

19.8

0.94

XGs/HMs/other

3,4,6p

- (−)

-

-

-

-

-

0.10

Other

2,3,4,6p

- (−)

-

-

-

-

0.45

0.15

Other

Galacturonic

terminalp

- (−)

0.12

1.30

1.21

-

-

0.05

HG/RG-II

acid

4p

9.21 (7.44)

19.1

70.79

54.42

12.1

8.29

6.22

HG/RG-I backbone

2,4p

- (−)

-

5.04

0.85

0.13

0.10

-

RG-II

3,4p

0.75 (0.91)

1.45

1.34

5.04

1.21

0.82

0.50

RG-II

4,6p

0.15 (0.40)

0.39

0.44

0.86

0.30

-

-

HG

Glucuronic

terminalp

7.10 (6.69)

0.28

-

-

5.31

0.44

-

AG-II/HXs/Other

acid

4p

1.14 (1.06)

1.23

-

-

0.49

0.63

1.74

AG-II/HXs/other

  1. Terminal GlcpA includes 4-O-Me GlcpA
  2. Values in parentheses represent HEPES fraction from upper segment of the collenchyma in celery petioles
  3. aTerminal Rhap deduced from 1,5-di-O-acetyl-6-deoxy-2,3,4-tri-O-methylrhamnitol, etc
  4. - Not detected