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Table 1 The yield, monosaccharide compositions (mol%) and degree of esterification of fractions and cell walls from collenchyma strands from different petiole segments, upper (U), middle (M) and lower (L)

From: Polysaccharide compositions of collenchyma cell walls from celery (Apium graveolens L.) petioles

Fractions

 

Yield (%)

Rha

Fuc

Ara

Xyl

Man

Gal

Glc

C-Glc

UA

TS (μg/mg)

DM

DA

HEPES

U

1.6a

2.5a

1.0a

9.7a

27.6a

2.3b

13.1a

9.2a

-

34.7a

338.7ab

16.9a

6.4a

M

1.7a

3.5b

0.7b

10.8b

22.2b

1.8a

13.7a

5.6b

-

41.7b

346.6b

15.6a

5.3a

L

1.5a

3.6b

0.7b

11.6b

20.1b

2.4b

15.0b

5.9b

-

40.7b

308.9a

19.5b

6.0a

CW

U

12.0a

2.5a (1.6a)

0.8a (1.0a)

4.4b (4.9b)

5.0a (8.0a)

1.6a (2.9a)

5.1b (5.7b)

3.4a (3.2a)

32.3a (30.4a)

44.9a (42.3a)

776.0a (819.7a)

25.9a

1.6a

M

11.4ab

2.0a (1.5a)

0.7a (0.9a)

3.3a (4.0a)

4.1a (7.6a)

1.5a (3.0a)

3.7a (4.7ab)

3.1a (2.9a)

37.4a (34.5c)

44.2a (40.8a)

766.0a (824.9a)

16.9b

1.4ab

L

9.5b

2.1a (1.4a)

0.7a (0.9a)

3.5a (3.9a)

4.5a (7.2a)

1.6a (3.0a)

3.6a (4.3a)

3.3a (3.1a)

34.9a (33.0b)

45.8a (43.3a)

766.6a (807.5a)

17.1b

1.2b

CDTA

U

16.6a

1.8a

Trace

5.0a

1.4b

Trace

2.3a

1.1a

-

88.4a

884.3a

3.8a

0.9a

M

16.9a

1.8a

Trace

4.3a

1.2a

0.3

2.0a

1.0a

-

89.3a

963.4a

3.2a

0.8a

L

20.5b

2.0a

Trace

4.4a

1.2a

0.3

2.1a

1.0a

-

89.1a

857.6a

5.3b

1.0a

Na2CO3

U

12.8a

3.7a

Trace

7.6a

1.9a

Trace

4.6a

1.0a

-

81.1b

587.2a

  

M

9.6a

4.6b

Trace

7.5a

2.1a

Trace

4.5a

1.3a

-

80.1b

509.8a

  

L

10.4a

5.1b

Trace

8.5a

2.0a

Trace

5.5a

2.3b

-

76.6a

572.0a

  

1 M KOH

U

13.1a

3.1a

1.6b

5.9a

22.3b

5.1a

8.7b

15.5a

-

37.8a

746.0a

  

M

9.0b

3.2a

1.0a

5.3a

19.3a

5.8a

7.0a

11.6a

-

46.8b

780.0a

  

L

10.5b

3.4a

1.4b

5.4a

20.7ab

5.3a

7.3a

13.8a

-

42.6ab

759.4a

  

4 M KOH

U

19.8a

3.8a

2.1a

5.9b

8.6a

5.8a

10.8a

20.1a

-

42.8a

717.8a

  

M

15.8a

2.8a

2.5a

4.0a

11.5b

5.7a

9.5a

25.8a

-

38.0a

747.5a

  

L

14.8a

3.6a

2.4a

4.8a

11.3b

5.2a

9.5a

23.6a

-

39.7a

723.0a

  

Residue

U

41.6a

1.9b (0.8b)

0.1a (0.3a)

2.8b (2.4b)

1.5a (2.4b)

0.7a (2.0a)

3.6b (3.0b)

8.3a (8.3a)

63.8a (63.8a)

16.8a (16.9a)

826.7a (831.6a)

  

M

36.4a

1.5ab (0.8b)

0.2a (0.3a)

2.0ab (2.0ab)

1.4a (2.4b)

0.7a (2.2a)

2.6b (2.5ab)

9.1a (8.9ab)

65.4a (64.1a)

17.0a (16.8a)

831.3a (845.9a)

  

L

33.8b

1.0a (0.5a)

0.1a (0.1a)

1.3a (1.5a)

1.5a (2.1a)

0.9b (2.3a)

1.8a (1.6a)

11.8b (11.6b)

70.2a (68.9a)

11.6a (11.4a)

819.7a (833.8a)

  
  1. The yield of HEPES fraction and cell walls means the freeze dried amount of them recovered from 100 g fresh collenchyma strands
  2. The yield of other fractions means the freeze dried amount of them recovered from 100 g freeze dried cell walls
  3. The values in bracket of final residue and cell walls means the amount of sugars hydrolysed by H2SO4; other values are from TFA hydrolysis
  4. “-” not detect. DM degree of methyl esterification of pectin (mol%), DA degree of acetyl esterification of cell wall (w/w%). Rha Rhamnose, Fuc fucose, Ara arabinose, xyl xylose, man mannose, Gal galactose, Glc non cellulosic glucose from TFA hydrolysis, C-Glc cellulose glucose, subtract TFA glucose from H2SO4, UA uronic acid, TS-total monosaccharides sum of uronic acid and neutral monosaccharides. The value averaged from duplicate or triplicate (n = 2 or 3)
  5. Different letters (a, b, c) indicate significant (P<0.05) differences between U, M and L