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Table 2 Proteins identified in L. incisa LB samples and selected for further analysis

From: Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa

  LB proteome Transcription [FPKM] LB localization
Protein name (accession) Average NSAF ± SD
[×10−3]
Coverage [%] Fold enrichment compared to
Total protein extract Membranes Soluble protein +N 3 d -N
LiMLDP (g555.p1) 4.9 ± 0.1 75.5 1.0 1.6 0.9 261.0 901.4 yes
LiLBP62 (g15430.p1) 3.2 ± 1.4 64.8 31.3 * 26.6 47.8 62.3 yes
LiLBP36 (g13945.p1) 4.5 ± 1.0 49.1 5.5 7.3 9.2 151.6 1656.1 yes
g9582.p1 2.9 ± 0.4 39.0 21.9 * 76.8 17.7 25.9 partial
g9864.p1 4.0 ± 1.4 50.4 2.1 1.5 2.5 47.2 805.4 no
g13714.p1 1.7 ± 0.2 45.1 7.9 3.0 12.8 24.9 77.0 no
g4703.p1 1.5 ± 0.3 37.3 2.2 25.6 7.3 122.5 94.3 no
g13209.p1 1.3 ± 0.1 46.9 3.9 2.0 5.1 55.2 428.7 no
g12144.p1 1.2 ± 0.2 28.7 4.2 3.9 5.8 15.8 119.0 no
g13747.p1 1.2 ± 0.2 37.0 8.0 25.7 12.8 17.6 25.2 no
g14373.p1 0.7 ± 0.3 21.2 24.8 * 57.6 57.4 158.9 no
  1. Only proteins that were detected in all three technical replicates were considered. Coverage refers to the proportion of the amino acid sequence covered by peptides detected in all samples. For each protein, fold enrichment in the LB sample compared to each control sample was calculated by division of the average Normalized Spectral Abundance Factors (NSAFs). Transcription levels in nitrogen replete conditions and after 3 d of nitrogen starvation were determined by RNA sequencing. LB localization was confirmed by heterologous expression in tobacco pollen tubes. SD, standard deviation of three technical replicates in a single experiment. FPKM, fragments per kilobase of transcript per million mapped reads. The asterisks denote samples, which did not contain the respective protein in all three technical replicates