Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa

BMC Plant Biology

Table 1 Filtering steps in the identification of possible LBPs based on high nanoLC-coupled mass spectrometry analysis

Filtering step	Number of proteins detected in LB samples
None	484 ± 107
Present in all 3 technical replicates	279
	>10× enriched compared to any control sample or exclusively present in LB sample	AND/OR most abundant 10% in LB sample	AND/OR expression induced >5× under nitrogen limiting conditions
	134	28	23
Selected for further analysis, coding sequence successfully amplified by RT-PCR	11
LB localization confirmed	3

Only proteins were considered, for which at least two peptides had been detected with medium or high confidence. For the initial number prior to filtering, the mean of three technical replicates is given ± the standard deviation

ISSN: 1471-2229