Fig. 4

Properties of LiLBP62. a Subcellular localization of LiLBP62-mVenus in N. tabacum pollen tubes. Following 6 h of pollen germination, cells were stained for LBs using Nile Red and fluorescence was documented by laser scanning microscopy. From top to bottom: Nile Red fluorescence, mVenus fluorescence, merged image. Scale bar = 10 μm. 5 out of 5 pollen tubes analyzed showed comparable results. The characteristic punctate pattern of the mVenus signal was also observed in 10 out of 10 unstained pollen tubes. b Hydrophobicity of amino acid positions in the sequence of LiLBP62 compared to A. thaliana OLEOSIN1. The hydrophobicity score was determined using ExPASy ProtScale software with the Kyte & Doolittle amino acid scale and a window size of 19 residues. c Changes in expression of LiLBP62 in response to varying nitrogen supply as determined by Illumina RNA sequencing. Samples from two L. incisa cultures were sequenced in 4 technical replicates each. The fold change of gene expression is given after 3 days of nitrogen starvation compared to nitrogen replete conditions and the significance of this change is given as the P value adjusted for multiple testing at a false discovery rate of 0.05. d Changes in expression of LiLBP62 in response to varying nitrogen supply as determined by qRT-PCR. Transcript levels were normalized to RIBOSOMAL PROTEIN S21. Expression is shown relative to time point 0 and error bars represent the standard error of the mean for three batches cultivated in parallel. The dotted line indicates the onset of nitrogen resupply and total fatty acid (TFA) levels are shown for comparison