Fig. 3
From: Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa
Heterologous expression of LiMLDP in the A. thaliana oleo1 mutant background. a Confocal laser scanning microscope images of LBs in the hypocotyls of isolated embryos are shown. T2 seeds were preselected for expression of the MCHERRY reporter gene and imbibed at 4 °C over night before embryo isolation and LB visualization by Nile Red staining. For each of 15 independent lines, three embryos were observed. Shown in the right panel is a result for line 3, which is representative for all lines analyzed. b Gene expression of LiMLDP is shown for all 15 independent lines in the upper panel; the mutant background was verified in the middle panel. Gene expression of AtACTIN8 served as control in the lower panel