Fig. 7

Phylogenetic comparison of P. vulgaris MYB amino acid sequences with known repressor and activator MYBs from other plant species. In silico translations of P. vulgaris MYB coding sequences corresponding to genes that were differentially expressed between darkening and non-darkening cranberry bean RILs were aligned to amino acid sequences of other plant MYBs using ClustalW (www.genome.jp/tools/clustalw; [81]). The maximum likelihood method in MEGA 6.06 was used to construct the unrooted tree [82]. Numbers proximal to each node represent the percent support values from the bootstrap analysis using 500 iterations. Previously characterized MYBs are represented in black font, and PvMYBs are represented in blue font. GenBank™ accession numbers for each MYB are provided in parentheses: Antirrhinum majus MYB MIXTA, AmMYB MIXTA (CAA55725.1); AmMYB-ROSEA2 (ABB83827.1); AmMYB-VENOSA (ABB83828.1); Arabidopsis thaliana MYB3, AtMYB3 (AAS10027.1); AtMYB4 (AAP13410.1); AtMYB5 (AEE75369.1); AtMYB12 (AEC10843.1); AtMYB16 (ABI49476.1); AtMYB32 (ABD91498.1); AtMYB75 (AAG09100.1); AtMYB90 (AEE34503.1); AtMYB123 (AAK54744.1); Diospyros kaki MYB2, DkMYB2 (BAI49719.1); DkMYB4 (BAI49721.1); Epimedium sagittatum MYB12, EsMYB12 (AFH03064.1); Fragaria x ananassa MYB1, FaMYB1 (AAK84064.1); FaMYB10 (ABX79947.1); Gossypium hirsutum MYB1, GhMYB1(AAN28270.1); Garcinia mangostana MYB10, GmMYB10 (ACM62751.1); Gentiana triflora MYB, GtMYB3 (BAF96933.1); GtMYBP3 (BAM71801.1); Leucaena leucocephala MYB1, LlMYB1(ADY38393.2); Lotus japonicus MYB TT2a, LjMYB-TT2a (BAG12893.1); LjMYB-TT2b (BAG12894.1); LjMYB-TT2c (BAG12895.1); Malus x domestica MYB10, MdMYB10 (ACQ45201.1); Medicago truncatula MYB, MtMYB (KEH30894.1); MtMYB2 (AES99346.1); MtMYB14 (AFJ53057.1); MtLAP1 (ACN79541.1); MtLAP2 (ACN79539.1); MtLAP3 (ACN79542.1); MtLAP4 (ACN79540.1); Prunus avium MYB, PaMYB (ADY15314.1); Picea glauca MYB4, PgMYB4 (ABQ51220.1); Petunia x hybrida AN2, PhMYB-AN2 (AAF66727.1); PhMYB1 (CAA78386.1); PhMYB4 (ADX33331.1); PhMYB PH4 (AAY51377.1); PhMYB-DPL (ADW94950.1); PhMYB27 (AHX24372.1); PhMYB-PHZ (ADW94951.1) Pinus taeda MYB4, PtMYB4 (AAQ62540.1); Populus tremula x Populus tremuloides, PtrMYB182 (AJI76863.1); Solanum lycopersicum MYB12, SlMYB12 (ACB46530.1); Sorghum bicolor Yellow Seed1, Sb-Yellow Seed1 (AAX44239.1); Theobroma cacao Transparent Testa 2-Like MYB, TcMYB-TT2 (ADD51352.1); Trifolium affine MYB14, TaMYB14 (AFJ53046.1); Trifolium arvense MYB14, TarMYB14 (AFJ53053.1); Trifolium occidentale MYB14, ToMYB14 (AFJ53052.1); Trifolium repens MYB4, TrMYB4 (AMB27079.1); TrMYB7 (AMB27080.1); TrMYB14 (AFJ53050.1); TrMYB133 (AMB27081.1); TrMYB134 (AMB27082.1); Tr RED LEAF (AIT76557.1); Tr RED LEAF DIFFUSEa (AIT76556.1); Tr RED LEAF DIFFUSEb (AIT76560.1); Tr REDVa (AIT76565.1); Vitis labrusca x Vitis vinifera MYBA1–1, VlMYBA1–1 (BAC07537.1); Vitis vinifera MYBPA1, VvMYBPA1 (CAJ90831.1); VvMYB4a (ABL61515.1); VvMYB5a (AAS68190.1); VvMYB5b (AAX51291.1); VvMYBC2 (ABW34393.1); VvMYBC2-L1 (AFX64995.1); VvMYBC2-L2 (ACX50288.1); VvMYBC2-L3 (AIP98385.1); VvMYB-PA2 (ACK56131.1); Zea mays MYB P1, ZmMYB P1 (ABM21535.1). In silico translations of cranberry bean MYBs were annotated as PvMYB1 to PvMYB15 and their corresponding gene accession numbers are provided in parentheses: PvMYB1 (Phvul.001G219000); PvMYB2 (Phvul.001G221500); PvMYB3 (Phvul.003G203900); PvMYB4 (Phvul.002G092100); PvMYB5 (Phvul.009G158200); PvMYB6 (Phvul.006G114800); PvMYB7 (Phvul.009G228200); PvMYB8 (Phvul.005G114100); PvMYB9 (Phvul.011G105600); PvMYB10 (Phvul.002G163500); PvMYB11 (Phvul.003G222400); PvMYB12 (Phvul.002G306000); PvMYB13 (Phvul.004G053600); PvMYB14 (Phvul.007G093100); PvMYB15 (Phvul.001G227900). Scale bar represents 0.2 amino acid substitutions per site