Fig. 3

Expression of A. thaliana ACL, AACT and HMGR in transgenic T. brevicorniculatum plants. a T-DNA constructs for the overexpression of AtACL(AB), AtAACT2 and Athmgrc1(S408A) in T. brevicorniculatum. NPTII, kanamycin resistance gene; BAR, bialaphos resistance gene; pMS, mannopine synthase promoter; pREF, rubber elongation factor promoter; LB, left border; RB, right border. b Protein extracts from the latex of 8-week-old transgenic and wild-type (Wt) plants analyzed by SDS-PAGE and western blot (upper part) with antibodies against the AtACLB subunit (α-ACL), AtAACT2 (α-AACT) and HMGR (α-HMGR), or Ponceau S staining after protein transfer (lower part). Numbers on the left refer to molecular weight markers. c Relative ACL, AACT and HMGR enzymatic activity in the latex of 8-week-old transgenic and wild-type plants. The values of the wild-type plants were set to 1. Bars represent standard errors of three independent transgenic plants per line. Normal distribution at p < 0.05 was assessed using the Kolmogorov-Smirnov test. Different letters indicate a significant difference (ANOVA with Tukey’s honest significant difference test (p < 0.01))