Fig. 2

Expression profile of clade V cucumber MLO genes in different tissues. a The relative transcript abundances of CsaMLO1, CsaMLO8 and CsaMLO11 in three tissues of the PM-susceptible cucumber cultivar ‘Sheila’ (hypocotyl, cotyledon and leaf) were determined using qRT-PCR. Data were normalized, using the geometric average of the Ct values of reference genes Ef-α and TIP41. Relative transcript abundances were calculated as 2^-dCt. Each bar shows the average transcript abundance of five to eight biological replicates on a logarithmic scale. The number of independent biological replicates per gene/tissue combination is given in the respective bars. Error bars indicate standard error of the mean. Different letters above bars indicate significant differences between genes (ANOVA with Bonferroni post hoc tests, P < 0.05). b The transcript abundances in seven tissues of cucumber (‘Chinese long’ inbred line 9930) were determined using RNA-seq. The FPKM values (Fragments Per Kilobase of transcript per Million mapped fragments) for CsaMLO1, CsaMLO8 and CsaMLO11 in each of these tissues are shown on a logarithmic scale. The amount of independent biological replicates per tissue was either one (hypocotyl, stem and cotyledon), two (root and root tip) or three (leaf and fruit). If applicable, error bars indicate the standard error of the mean. Different letters above bars indicate significant differences between genes (ANOVA with Bonferroni (for fruit tissue) or Dunnet T3 (for leaf tissue) post hoc tests, P < 0.05)