| Non-stressed (NS) lines | Stressed (S) lines | Background (restriction site) | Events and their quantification | Events affecting CG/CHG sequence methylation status change | ||||
---|---|---|---|---|---|---|---|---|---|
Digest/MSAP profile | NS (5′-3′/3′-5′) | S (5′-3′/3′-5′) | NS - > S | ||||||
4-digit code/events | EcoRI/HpaII | EcoRI/MspI | EcoRI/HpaII | EcoRI/MspI | |||||
0000 | 0 | 0 | 0 | 0 | mCmCGG GGCmCm | mCmCGG GGCmCm | MM/MM-> MM/MM | MSPex × 2; MSPin × 2 |  |
MM/MM- > M−/−M | MSPex × 2; DMin × 2 | DM-CG × 2 | |||||||
mCCGG GGCCm | mCCGG GGCCm | M−/−M- > M−/−M | MSPex × 2; NMSPin × 2 |  | |||||
M−/−M- > MM/MM | MSPex × 2; DNMin × 2 | DNM-CG × 2 | |||||||
0001 | 0 | 0 | 0 | 1 | mCmCGG GGCmCm | CmCGG GGCC | MM/MM- > −M/−− | DMex × 2; DMin; MSPin | DM-CHG × 2; DM-CG |
MM/MM- > −M/M- | DMex × 2; MSPin × 2 | DM-CHG × 2; | |||||||
mCCGG GGCCm | CmCGG GGCmC | M−/−M- > −M/−− | DMex × 2; DNMin; NMSPin | DM-CHG × 2; DNM-CG | |||||
M−/−M- > −M/M- | DMex × 2; DNMin × 2 | DM-CHG × 2; DNM-CG × 2 | |||||||
0010 | 0 | 0 | 1 | 0 | mCmCGG GGCmCm | mCCGG GGCC | MM/MM - > M−/−− | DMex; DMin × 2; MSPex | DM-CHG; DM-CG × 2 |
mCCGG GGCCm | M−/−M- > M−/−− | DMex; NMSPin × 2; MSPex | DM-CHG | ||||||
0011 | 0 | 0 | 1 | 1 | mCmCGG GGCmCm | CCGG GGCC | MM/MM- > −−/−− | DMex × 2; DMin × 2 | DM-CHG × 2; DM-CG × 2 |
mCCGG GGCCm | M−/−M- > −−/−− | DMex × 2; NMSPin × 2 | DM-CHG × 2 | ||||||
0100 | 0 | 1 | 0 | 0 | CmCGG GGCC | mCmCGG GGCmCm | -M/−− − > MM/MM | DNMex × 2; DNMin; MSPin | DNM-CHG × 2; DNM-CG |
-M/−− − > M−/−M | DNMex × 2; DMin; NMSPin | DNM-CHG × 2; DM-CG | |||||||
CmCGG GGCmC | mCCGG GGCCm | -M/M- - > MM/MM | DNMex × 2; MSPin × 2 | DNM-CHG × 2 | |||||
-M/M- - > M−/−M | DNMex × 2; DMin × 2 | DNM-CHG × 2; DM-CG × 2 | |||||||
0101 | 0 | 1 | 0 | 1 | CmCGG GGCC | CmCGG GGCC | -M/−− − > −M/−− | NMSPex × 2; MSPin; NMSPin |  |
-M/−− − > −M/M- | NMSPex × 2; MSPin; DNMin | DNM-CG | |||||||
CmCGG GGCmC | CmCGG GGCmC | -M/M- - > −M/−− | NMSPex × 2; MSPin; DMin | DM-CG | |||||
-M/M- - > −M/M- | NMSPex × 2; MSPin × 2 |  | |||||||
0110 | 0 | 1 | 1 | 0 | CmCGG GGCC | mCCGG GGCC | -M/−− − > M−/−− | DNMex; DMin; NMSPex; NMSPin | DNM-CHG; DM-CG |
CmCGG GGCmC | -M/M- - > M−/−− | DNMex; DMin × 2; NMSPex | DNM-CHG; DM-CG × 2 | ||||||
0111 | 0 | 1 | 1 | 1 | CmCGG GGCC | CCGG GGCC | -M/−− − > −−/−− | NMPex × 2; DMin; NMSPin | DM-CG |
CmCGG GGCmC | -M/M- - > −−/−− | NMSPex × 2; DMin × 2 | DM-CG × 2 | ||||||
1000 | 1 | 0 | 0 | 0 | mCCGG GGCC | mCmCGG GGCmCm | M−/−− − > MM/MM | MSPex; DNMex; DNMin × 2 | DNM-CHG; DNM-CG × 2 |
mCCGG GGCCm | M−/−− − > M−/−M | MSPex; DNMex; NMSP × 2 | DNM-CHG | ||||||
1001 | 1 | 0 | 0 | 1 | mCCGG GGCC | CmCGG GGCC | M−/−− − > −M/−− | DMex; DNMin; NMSPex; NMSPin | DM-CHG; DNM-CG |
CmCGG GGCmC | M−/−− − > −M/M- | DMex; DNMin; NMSPex; DNMin | DM-CHG; DNM-CG × 2 | ||||||
1010 | 1 | 0 | 1 | 0 | mCCGG GGCC | mCCGG GGCC | M−/−− − > M−/−− | MSPex; NMSPin × 2; NMSPex |  |
1011 | 1 | 0 | 1 | 1 | mCCGG GGCC | CCGG GGCC | M−/−− − > −−/−− | DMex; NMSPin × 2; NMSPex | DM-CHG |
1100 | 1 | 1 | 0 | 0 | CCGG GGCC | mCmCGG GGCmCm | −−/−− − > MM/MM | DNMex × 2; DNMin × 2 | DNM-CHG × 2; DNM-CG × 2 |
mCCGG GGCCm | −−/−− − > M−/−M | DNMex × 2; NMSPin × 2 | DNM-CHG × 2 | ||||||
1101 | 1 | 1 | 0 | 1 | CCGG GGCC | CmCGG GGCC | −−/−− − > −M/−− | NMSPex × 2; DNMin; NMSPin | DNM-CG |
CmCGG GGCmC | −−/−− − > −M/M- | NMSPex × 2; DNMin × 2 | DNM-CG × 2 | ||||||
1110 | 1 | 1 | 1 | 0 | CCGG GGCC | mCCGG GGCC | −−/−− − > M−/−− | NMSPin × 2; NMSPex; DNMex; | DNM-CHG |
1111 | 1 | 1 | 1 | 1 | CCGG GGCC | CCGG GGCC | −−/−− − > −−/−− | NMSPex × 2; NMSPin × 2 |  |