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Fig. 1 | BMC Plant Biology

Fig. 1

From: Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Fig. 1

Temporal ROS levels during anther development in tobacco and tomato. aj Representative NBT staining of tobacco (ae) or tomato (fj) anthers during development. In total, 20–25 anthers at each development stage from three independent batches of plants were analyzed and similar results were obtained. k Relative ROS levels in tobacco or tomato anthers at each developmental stage based on H2DCF-DA staining of ROS. Results shown are means + standard deviation (SD, N = 20). a.u., arbitrary fluorescent unit. Classification of anther groups is based on anther sizing described in Additional file 1: Table S1. Bars, 800 μm for (ae); 1000 μm for (fj)

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