Fig. 3

Skewing of tno1 roots is enhanced upon MT stabilization by taxol. a Angle of skew with respect to the horizontal for WT, KO and COM roots grown on two different concentrations of taxol. Seedlings were grown vertically in LD conditions for 5 days on growth media solidified with 1.5% agar and either containing taxol or solvent as a control. All values represent the means of three biological replicates with at least 12–15 seedlings for each replicate. Error bars indicate standard error. Asterisks indicate a statistically significant difference (P < 0.05) by Student’s t-test. b Light microscopic images of roots of WT, KO and COM lines grown vertically in the presence of 0.2 μM taxol for 5 days. Scale bar =1 cm. c Mean root skew of each genotype on taxol compared to the mean on the solvent control was expressed as percentage increase in skew. Values represent analysis of 3 biological replicates with at least 12 seedlings for each replicate. Error bars indicate standard errors. Different letters indicate statistically significant differences (P < 0.05) by Student’s t-test. d Confocal images of propidium iodide-stained elongation zones of WT, KO and COM roots displaying epidermal CFR. The roots were grown vertically on 0.2 μM taxol for 5 days. Scale bar = 100 μm. e Cell file rotation angles of skewing roots of WT, KO and COM lines grown on 0.2 μM taxol. All values represent the means of 3 biological replicates with at least 10 cell files from 3 seedlings analyzed for each replicate. Error bars indicate standard errors. Asterisks indicate a statistically significant difference (P < 0.01) by Student’s t-test