Fig. 4From: Acetolactate synthase regulatory subunits play divergent and overlapping roles in branched-chain amino acid synthesis and Arabidopsis development AIP1 and AIP3 are essential for Arabidopsis growth and development. a Schematic representation of the T-DNA insertion aip1-1, aip1-2, and aip3-1 mutants. b Assessment of expression of AIP1 and AIP3 in aip1-2 and aip3-1 knockout lines when using RT-PCR. c Phenotypes of 9-day-old wild type, single and double aip knockout mutants. The aip1-2/aip3-1 double knockout mutant, Col-0 and the single aip knockout mutants grown on control media (0.5× MS), only the double aip knockout mutants exhibit a synthetic lethal phenotype, then fail to develop adult organs followed by chlorosis and deathBack to article page