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Fig. 3 | BMC Plant Biology

Fig. 3

From: Acetolactate synthase regulatory subunits play divergent and overlapping roles in branched-chain amino acid synthesis and Arabidopsis development

Fig. 3

AIP1 and AIP3 localize to both the chloroplast and peroxisome. a Subcellular localization of C-terminal YFP-tagged fusions of ALS, AIP1, and AIP3 in N. benthamiana. ALS-, AIP1-, and AIP3-YFP were expressed in Nicotiana leaves and analyzed by confocal microscopy. The localization of the AIPs and ALS are visualized by yellow fluorescence. Red fluorescence: excited chlorophyll emission to mark plastid location. YFP-ALS arrowheads: stromal localization of C-terminal YFP-tagged fusion, YFP-ALS. AIP3-YFP arrowheads: localization of AIP3-YFP outside of chloroplasts. Scale Bar, 20 μm (b) Subcellular localization of ALS, AIP1, AIP3, N-terminal YFP tagged fusions in Nicotiana leaves along with a peroxisomal marker (Px-CFP). Red fluorescence: excited chlorophyll emission to mark plastid location. Arrowheads: co-localization of Px-CFP with AIP1-YFP and AIP3-YFP

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BMC Plant Biology

ISSN: 1471-2229

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