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Fig. 3 | BMC Plant Biology

Fig. 3

From: An integrated RNAseq-1H NMR metabolomics approach to understand soybean primary metabolism regulation in response to Rhizoctonia foliar blight disease

Fig. 3

1H NMR metabolite partial least squares score and loadings plots 12 and 24 h.p.i. Partial least squares-discriminant analysis (PLS-DA) PC1/PC2 score (a and b) and loadings (c and d) plots of 1H NMR metabolite profiles 12 h.p.i. (a and c) and 24 h.p.i. (b and d) of R. solani AG1-IA infected (squares) and mock-inoculated control (circles) soybean leaves. Ellipses represent Hotelling T2 with 95% confidence intervals. Metabolites to the left increased during R. solani disease progression, while those on the right decreased in response to RFB compared to controls. Six biological replicates were analyzed per treatment per time point. Q2(cum), cumulative fraction of the total variation of the X’s that can be predicted by the components; R2X and R2Y, the fraction of the sum of squares of all X’s and Y’s explained by the components, respectively. Aln, alanine; D-gluc, D-glucose; EtOH, ethanol; For, formate; Fum, fumarate; Fruc, fructose; GABA, γ-aminobutyrate; Gln, glutamine; Glu, glutamate; Gluc, glucose; Phe, phenylalanine; Pro, proline; Suc, succinate; Sucr, sucrose; Tyr, tyrosine

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