Fig. 5

Tre6P inhibitable in vitro SnRK1 activity for control and sucrose-starved kernels (2012 season). SnRK1 activity was determined in tissue extracts as described by [20]. Exogenous Tre6P (1 mM) was added to determine the fraction of SnRK1 activity sensitive to Tre6P inhibition. Shaded region of graph indicates starvation period for treated kernels, and unshaded region indicates recovery period. Control samples are indicated with filled squares (black) and starved kernels are indicated with open squares (white). Asterisks indicate significant difference from controls (P < 0.05; n = 3). Standard error is shown with vertical bars