Fig. 3

Xyloglucan motifs recognised by CCRC-M1 and LM15 mAbs are specific to the CFML. a All micrographs are cross sections of fibre tissue of the Coker variety (G. hirsutum). Calcofluor staining of the LM15 labelled sections are shown on the left-hand side. The fucosylated and XXXG motifs of xyloglucans (CCRC-M1 and LM15 panels) are prominent in the CFML bulges while these were less abundant in the cotton fibre cell wall where galactosylated xyloglucans appeared homogeneously distributed (LM25 panels). The paired CFML bulges, relevant during the transition phase from elongation to secondary cell wall deposition, are not depicted by the de-esterified HG epitope (LM19 panels). b 10 dpa cross sections of the G. barbadense PimaS7 and the G. hirsutum FM966 cultivars showing the presence of fucosylated xyloglucans in the CFML (arrowheads). c 15 dpa cross section of the G. hirsutum FM966 cultivar treated with xyloglucanase removed xyloglucan (arrowheads in right panel - LM15) but did not affect the staining of the CFML with Calcofluor White (arrowheads in left panel) indicating the presence of other β-glucans not characterized yet. Scale bars =10 μm. All images in each panel are at the same magnification