Fig. 7

Concordance between the abundance of intermediates in the biosynthesis of Raffinose Family Oligosaccharides (RFO) and the transcripts encoding the enzymes of the pathway during the dehydration-rehydration cycle in young leaf tissues of S. stapfianus. A single longitudinal bar directly under the compound in the pathway represents the metabolite abundance heat map for each compound. The data used to generate the metabolite heat maps are reported in Oliver et al 2011. Red shading indicates a statistically significant fold increase in abundance and green shading a statistically significant fold decrease in metabolite abundance. White indicates no change in metabolite abundance. For the dehydrating samples (60% RWC to DRY) the fold change is given in relation to the hydrated control, where red shading indicates a statistically significant increase in abundance compared to the hydrated control for the dehydrating samples. For the rehydrating samples the fold change is given in relation to either the dry samples (/D) or the hydrated control (/H). The longitudinal bars directly associated with the enzyme identity in the pathway represent the transcript abundance heat map for each enzyme. Each line in the heat map constitutes a single contig annotated as encoding the associated enzyme. Red shading indicates a statistically significant the log₂-fold increase in abundance and green shading a statistically significant the log₂-fold decrease in abundance. Black indicates no change in transcript abundance. For the dehydrating samples (80% RWC to DRY) the log₂-fold change in abundance is given in relation to the hydrated control. For the rehydrating samples log₂-fold change in abundance is given in relation to either the dry samples (/DRY) or the hydrated control (/HYD)