Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Fig. 3

Stability of the BP178 peptide accumulated in 3 years-old seeds. The endosperm tissue was manually dissected from mature seeds (0-days) and germinating rice seeds (3 and 7 days of germination). Protein extracts (35 μg) were separated by Tricine-SDS gels, transferred onto PVDF membrane, and incubated with the anti-BP178 antibody followed by anti-rabbit IgG (Fc) alkaline phosphatase conjugated secondary antibody. EV, rice plants with the empty pCAMBIA 1300 vector were used as a negative control, and 3.2 μg of synthetic BP178 were used as reference control. Arrows, indicate BP178 A-related bands in rice extracts. Numbers in the left side indicate the size of the molecular markers (kDa). Lower panels show Ponceau staining of protein samples

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us