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Fig. 2 | BMC Plant Biology

Fig. 2

From: Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Fig. 2

BP178 peptide accumulates in the endosperm of Glb1:BP178 transgenic rice seeds without altering accumulation levels of native storage proteins. a Coomassie Blue staining of PB extracts from the indicated seeds. EV indicates seeds from plants transformed with the pCAMBIA 1300 empty vector. b Ponceau staining of protein samples (50 μg) c Western-blot analysis and quantification of the BP178 peptide in the protein body-enriched fractions (50 μg) from mature seed endosperms of the indicated pGlb1:BP178, pGluB4:BP178, pGluB1:BP178 and empty vector (EV) lines. The accumulation levels of BP178 peptide were quantified with the Quantity tools of the Chemidoc program using the 0.3, 0.15 and 0.05 μg amounts of synthetic BP178 peptide as a reference (taking into account the sum of the three bands observed). Numbers below the western blot lanes correspond to μg of BP178 peptide. d Western-blot analysis of protein body-enriched fractions (50 μg) from embryos, leaves or roots of the indicated pGlb1:BP178, and empty vector (EV) lines. For comparative purposes, 0.30, 0.15 and 0.05 μg of synthetic BP178 peptide were run simultaneously in the Tricine-SDS gels. Immunodetection was performed using specific polyclonal anti-BP178KDEL antibodies and coloured phosphatase alkaline reaction

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