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Table 2 List of methylated proteins found in E. grandis root tissues with elevated expression of EgPRMT1 or EgPRMT10

From: Root morphogenic pathways in Eucalyptus grandis are modified by the activity of protein arginine methyltransferases

 

Protein

Gene No.

Score

Methylated Peptide Sequences Identified by Mass Spectrometry

Predicted MW (kDa)

35S::PRMT1

Endoplasmin/Heat Shock protein 90 kDa

Eucgr.H00972.1

6111, 162.292

531 LGIIEDASNR

93

RuBisCO large subunit- binding protein alpha

Eucgr.B02532.1

2181, 89.942

424 VGAATESELEDR

69

Alpha tubulin

Eucgr.B03604.1

851, 92.432

65 AIFLDLEPTVVDEVR

49

Glyceraldehyde-3-phosphate dehydrogenase

Eucgr.H04673.1

4991, 164.682

66 SISISISISIRPVR 346 RLEKAATYDEIK

36

Probable aldo-keto reductase

Eucgr.H04367.1

79.322

249 NRVVYAR

38

Armadillo/beta catenin repeat protein

Eucgr.B00340.1

21.522

377 IKLVKLNAVSALLGMLKSR

58

40S Ribosomal Protein

Eucgr.A02371.1

1171

194 VLQFAGIDDVFTSSR

29

Transaldolase family protein

Eucgr.G01978.1

2771

78 RTTLHDLYER

47

Probable Aldo-keto reductase

Eucgr.H04365.1

1062

106 GTPEYAR

38

ATP synthase subunit beta

Eucgr.G02224.1

1221

335 DAEGQDVLLFIDNIFR

60

Beta tubulin 5

Eucgr.D01847.1

4811

155 IREEYPDR

50

Elongation factor 1-alpha

Eucgr.B02473.1

1791

244 IGGIGTVPVGR

49

Phosphoenolpyruvate carboxylase

Eucgr.A01915.1

139.72

446 RLDIR

110

Heat shock protein 80 kDa

Eucgr.J00025.1

13411, 211.262

131 NRTTPSYVAFTDTER 558 FELSGIPPAPR

80

Sucrose Synthase

Eucgr.H03515.1

5361, 133.92

772 HVSNLDR

92

35S::PRMT10

Endochitinase A2

Eucgr.I01495.1

2131, 127.562

107 SFPAFGTTGDAATR

33

Beta Tubulin 5

Eucgr.D01847.1

1481

155 IREEYPDR

50

Elongation factor 1-alpha

Eucgr.B02473.1

4981

165 ARYDEIVK

49

Heat shock protein 70 kDa

Eucgr.J00023.1

3821, 204.212

38 NRTTPSYVAFTDTER 478 DNNLLGKFELSGIPPAPR, 540 TTPSYVGFTDTER

69

  1. List of proteins identified from bands of whole cell lysates that correspond to methylated banding as determined by Western blotting. Score determined by either Mascot (1) or Peaks (−10logP) (2). Methylated arginines within sequences indicated in bold (dimethylated) or underlined (monomethylated)