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Fig. 3 | BMC Plant Biology

Fig. 3

From: Root morphogenic pathways in Eucalyptus grandis are modified by the activity of protein arginine methyltransferases

Fig. 3

Effect of PRMT methylation inhibitor treatment on root morphology. a Rate of root growth of roots after 24 h of treatment with methylation inhibitors AdOx (5 μM white bars, 0.5 μM light grey bars, 50 nM dark grey bars, 5 nM black bars), AMI-1 (50 μM white bars, 5 μM light grey bars, 0.5 μM dark grey bars, 50 nM black bars) and 2,3-dimethoxynitrostyrene (DMNS; 30 μM white bars, 3 μM light grey bars, 0.3 μM dark grey bars, 30 nM black bars in 1% DMSO) or controls (water hatched bar or 1% DMSO cross hatched bar). b ASYM24 probed Western blot of total protein extracted from control or PRMT inhibitor treated roots. c Representative photos showing the morphology of roots treated with methylation inhibitors or controls. Arrows indicate abnormal root bulging observed in AdOx and AMI-1 treated roots. d Size of the root meristem after treatment with the highest concentration of PRMT inhibitor (dark grey bars) as compared to control treated roots (black bars). e Root elongation zone size after treatment with the highest concentration of PRMT inhibitor (light grey bars) as compared to control treated roots (black bars). ± SE; * = significant difference from corresponding control treatment (p < 0.05)

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