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Fig. 4 | BMC Plant Biology

Fig. 4

From: Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Fig. 4

Water permeability in P. pastoris spheroplasts. Stopped-flow traces showing kinetics of osmotic water permeability in spheroplasts with empty pPICZB plasmid (black), spheroplasts expressing NbXIP1;1αwt (red), spheroplasts expressing NbXIP1;1αL79G/I102H/V242I (blue) and spheroplasts expressing NbXIP1;1αL79G/I102H/T246I (green). The traces (mean of at least 15 traces) were fitted to single exponential equations. The mean rate constants ± standard deviations for the fitted curves were: 2.19 ± 0.25 s−1 (Empty); 2.20 ± 0.42 s−1 (NbXIP1;1αwt); 5.52 ± 1.21 s−1 (NbXIP1;1αL79G/I102H/V242I) and 2.84 ± 0.48 s−1 (NbXIP1;1αL79G/I102H/T246I)

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