Fig. 2
From: Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable
Alternative alignments of helix 5 in NbXIP1s. Multiple sequence alignments showing two possible helix 5 alignments of NbXIP1s with sequences of AQPs with solved crystal structures. The H5P, LEP and HEP positions representing the helix 5, loop E and helix E positions respectively of the aromatic arginine selectivity filter are shown in black boxes. a ICVAR alignment aligns the two glycines (asterisk) in the helix 5 of NbXIP1s with the two glycines in the helix 5 of AtTIP2;1. This alignment places valine 242 of NbXIP1;1α at the H5P position of the aromatic arginine selectivity filter. b ICTAR alignment aligns only one of the glycines in the helix 5 of NbXIP1s with the conserved glycine in the helix 5 of the AQPs with solved structures. This alignment places threonine 246 of NbXIP1;1α at the H5P position of the aromatic arginine selectivity filter