Fig. 3

Ribosomal protein GaRPL18 is involved in the resistance of cotton to V. dahliae. a Disease symptoms induced with V. dahliae strain on the leaves of empty-vector control plants and CLCrV:GaRPL18 plants at 25 dpi. b 25-dpi stem sections were plated on PDA medium, incubating at 25 °C. Photos were taken at 7 day after plating. c Transcript levels of GaRPL18 in leaves of empty-vector control plants and CLCrV:GaRPL18 plants, which were determined by qRT-PCR using Gahiston3 as the internal reference gene. Value of CLCrV was normalized to ‘1’. d Assessment of Disease Index (DI) at 20 dpi and 25 dpi. Error bars represent the standard deviation of three biological replicates (n ≥ 30). e The levels of Verticillium dahliae biomass by qRT-PCR on the leaves of empty-vector control plants and CLCrV:GaRPL18 plants at 20 dpi and 25 dpi. Error bars represent the standard deviation of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P <0.05; **P <0.001). f The infected CLCrV:GaRPL18 plants showed vascular browning in xylem at 25 dpi