Fig. 1

Structural analysis and expression patterns of GaRPL18 under Verticillium dahliae stress or hormone treatment in cotton. a Gene structure analysis. b Protein 3D structure. c,d After seeding for 3 weeks, the cotton seedlings of ‘HuNanChangDeTieZiMian’ (resistant, Ga01) and ‘NaShangQuXiaoHua’ (susceptible, Ga02) were inoculated with V. dahliae spores or an equal sterile distilled water (Mock control). The sample of c is the leaf, while d is root. e The leaves of resistant cotton (‘HuNanChangDeTieZiMian’) were treated by exogenous hormones or an equal sterile distilled water. The Mock and treated roots and leaves were harvested at 0, 6, 12, 24, 48, and 72 hpi, and expression levels were determined by qRT-PCR using Gahiston3 as the internal reference gene. Expression levels of Mock control was normalized to ‘1’, error bars represent the standard deviation of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P <0.05; **P <0.001)