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Table 3 Two-way ANOVA and multiple linear regression analysis (MLR) of the relative gene expression in Brassica juncea seedlings raised from EBR-soaked seeds and grown in IMI supplemented substratum

From: 24-epibrassinolide stimulates imidacloprid detoxification by modulating the gene expression of Brassica juncea L

Gene name

F-ratios

HSD (p < 0.05)

FIMI

FEBR

FIMI×EBR

SOD

0.78

48.57***

0.01

0.26

CAT

16.66**

87.07***

6.89*

0.45

GR

7.05*

124.34***

42.83***

0.41

RBOH

19.06**

0.46

31.10***

0.66

NADH

4.16

51.72***

18.29**

0.46

CXE

21.64**

88.35***

2.26

0.56

GSH-S

0.93

10.57*

2.86

0.46

GSH-T

0.59

17.10**

1.14

0.60

GST-1

21.14**

26.40***

8.68*

0.40

GST-2

6.86*

143.74***

0.52

0.49

GST-3

12.82**

99.63***

1.85

0.90

GST-4

0.09

8.38*

1.07

0.43

GST-5

0.19

110.10***

0.11

1.06

GST-6

0.059

143.70***

5.28

1.21

POD

40.71***

23.44**

1.66

1.24

P450

11.03*

51.62***

4.06

0.96

MLR equation

β-regression coefficient

Multiple correlation coefficient

Significant at p <

βIMI

βEBR

SOD expression = 0.9960 + 0.0003 X1 + 0.0041 X 2

0.1169

0.9201

0.9274

0.001

CAT expression = 1.1311–0.0020 X1 + 0.0093 X 2

– 0.3748

0.8566

0.9351

0.001

GR expression = 1.3034 + 0.0012 X1 + 0.0103 X 2

0.1968

0.8260

0.8491

0.001

RBOH expression = 1.4136 + 0.0032 X1–0.0010 X 2

0.5702

– 0.0892

0.5771

0.10

NADH expression = 1.2212 + 0.0011 X1 + 0.0074 X 2

0.2250

0.7933

0.8246

0.01

CXE expression = 0.9051 + 0.0029 X1 + 0.0118 X 2

0.4242

0.8571

0.9563

0.001

GSH-S expression = 0.9113 + 0.0005 X1 + 0.0034 X 2

0.2040

0.6875

0.7170

0.02

GSH-T expression = 1.0713 + 0.0005 X1 + 0.0055 X 2

0.1483

0.7983

0.8120

0.01

GST-1 expression = 0.8681 + 0.0021 X1 + 0.0046 X 2

0.5737

0.6411

0.8604

0.001

GST-2 expression = 1.0400 + 0.0015 X1 + 0.0133 X 2

0.2077

0.9504

0.9728

0.001

GST-3 expression = 0.8635 + 0.0036 X1 + 0.0200 X 2

0.3238

0.9025

0.9588

0.001

GST-4 expression = 0.9497 + 0.0002 X1–0.0030 X 2

0.0747

– 0.6909

0.6949

0.02

GST-5 expression = 1.0393 + 0.0005 X1 + 0.0248 X 2

0.0401

0.9643

0.9651

0.001

GST-6 expression = 1.3087 + 0.0003 X1 + 0.0322 X 2

0.0195

0.9565

0.9567

0.001

POD expression = 1.1777 + 0.0088 X1 + 0.0134 X 2

0.7426

0.5635

0.9322

0.001

P450 expression = 1.2155–0.0036 X1 + 0.0154 X 2

– 0.3842

0.8312

0.9157

0.001

  1. X1 = IMI (mg L−1), X 2 = EBR (nM), *, ** and *** = significant at p < 0.05, p < 0.01 and p < 0.001 respectively
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BMC Plant Biology

ISSN: 1471-2229

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