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Table 2 Methyl-accepting assay of endosperm and leaf DNA

From: Inter-individual variation in DNA methylation is largely restricted to tissue-specific differentially methylated regions in maize

  

Target/haploid genome (x106)a

Methylase

Target sequenceb

Endospermc

Leafc

HpaII

CCGG

0.34 ± 0.12

0.071 ± 0.016

MspI

CCGG

0.64 ± 0.24

0.16 ± 0.024

SssI

CG

1.94 ± 0.51

0.41 ± 0.25

  1. aMean ± SD; n = 6 and n = 5 of endosperm and leaf, respectively
  2. bUnderlined cytosine indicates target of methylase
  3. cAll differences between groups (i.e. between tissues with same enzyme treatment and between enzyme treatments within tissues) were statistically significant (p < 0.05)
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BMC Plant Biology

ISSN: 1471-2229

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