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Fig. 4 | BMC Plant Biology

Fig. 4

From: Inter-individual variation in DNA methylation is largely restricted to tissue-specific differentially methylated regions in maize

Fig. 4

Expression profiles of genes harbouring variable and non-variable HpaII sites. a transcription data of mixed seedling V1 stage (L) and 14 DAP endosperms (E) from ZM37 (Plant Expression Database); the dotted line represents the 7.6 threshold value commonly used for presence of expression calling [52]; L ~ E refers to genes that contained HpaII sites that were unmethylated (detected) in either tissue, while L > E and L < E refer to HpaII sites that were more or less methylated in leaf relative to endosperm, respectively (as determined by comparing endosperm and leaf MSAP profiles; see Additional file 4: Figure S2a); gene-body was defined as the region between transcriptional start and termination sites (TSS and TTS, respectively); asterisks indicates significant differences in expression between leaf and endosperm tissues (* = p < 0.05 and bl = borderline significance, p < 0.1); b left panel: RT-PCR expression profiles of three genes across leaf (L) and endosperm (E) tissues; + and – indicates presence or absence of reverse transcriptase, respectively; right panel: positions of variable HpaII sites (open lollipops) relative to the region amplified by RT-PCR (double-headed arrows)

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