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Fig. 4 | BMC Plant Biology

Fig. 4

From: Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) gene from the microalga Chlorella ellipsoidea

Fig. 4

Nile Red staining and TLC analysis of transformed yeast strain H1246. (A) The formation of lipid bodies is restored upon the expression of CeDGAT1 in the yeast strain H1246. Nile Red staining (left) and interference contrast images (right) of yeast. The wild-type INVSc1 harbouring the empty vector was used as a positive control (a). The mutant strain H1246 harbouring the empty vector was analysed as a negative control (b). The TAG-deficient quadruple mutant strain H1246 expressing CeDGAT1 (c). Bar = 2.5 μm. (B) TLC analysis of lipid extracts from yeast expressing CeDGAT1. (1) TAG marker; (2) The mutant strain H1246 harbouring the empty vector analysed as a negative control; (3) Mutant strain H1246 expressing CeDGAT1; and (4) The wild-type strain INVSc1 harbouring the empty plasmid used as a positive control. SE, steryl ester; TAG, Triacylglycerol; FFA, free fatty acid; DAG, diacylglycerol

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BMC Plant Biology

ISSN: 1471-2229

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