Metabolic pathways | Metabolites name | Major metabolites in roots | Major metabolites in shoots | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Relative concentration | Fold changes | Relative concentration | Fold changes | ||||||||
CK | SS | AS | Log2 (SS/CK) | Log2 (AS/CK) | CK | SS | AS | Log2 (SS/CK) | Log2 (AS/CK) | ||
TCA cycle | Citric acid | 46.97 ± 3.85 | 32.51 ± 2.26 | 94.78 ± 5.39 | −0.53 | 1.01* | 3.66 ± 0.38 | 1.99 ± 0.10 | 5.24 ± 0.62 | −0.88* | 0.52 |
Aconitic acid | 68.74 ± 4.73 | 80.81 ± 5.04 | 136.35 ± 8.75 | 0.23 | 0.99* | 0.50 ± 0.03 | 0.36 ± 0.02 | 0.83 ± 0.06 | −0.47 | 0.73 | |
α-Ketoglutaric acid | 0.31 ± 0.02 | 0.20 ± 0.00 | 0.26 ± 0.01 | −0.62 | −0.27 | 1.48 ± 0.04 | 0.42 ± 0.01 | 0.97 ± 0.05 | −1.82* | −0.60 | |
Succinic acid | 7.60 ± 0.83 | 10.58 ± 0.29 | 41.12 ± 3.06 | 0.48 | 2.44* | 4.59 ± 0.27 | 2.76 ± 0.38 | 3.29 ± 0.64 | −0.73 | −0.48 | |
Fumaric acid | 1.09 ± 0.04 | 0.83 ± 0.01 | 3.08 ± 0.19 | −0.39 | 1.51* | 0.50 ± 0.04 | 0.36 ± 0.02 | 0.44 ± 0.07 | −0.48 | −0.20 | |
Malic acid | 9.90 ± 0.95 | 6.76 ± 0.38 | 22.15 ± 1.75 | −0.55 | 1.16* | 66.10 ± 5.03 | 39.87 ± 2.36 | 45.92 ± 4.69 | −0.73* | −0.53 | |
Glycolysis | Glucose | 1.58 ± 0.09 | 0.37 ± 0.01 | 0.28 ± 0.00 | −2.11* | −2.49* | 0.06 ± 0.00 | 0.07 ± 0.00 | 0.04 ± 0.00 | 0.23 | −0.65 |
Glucose-6-P | 0.81 ± 0.03 | 0.18 ± 0.00 | 0.68 ± 0.05 | −2.15* | −0.26 | 0.16 ± 0.00 | 0.46 ± 0.03 | 0.06 ± 0.00 | 1.48* | −1.40* | |
Fructose-6-P | 0.53 ± 0.07 | 0.12 ± 0.00 | 0.43 ± 0.15 | −2.08* | −0.28 | 0.14 ± 0.00 | 0.61 ± 0.03 | 0.10 ± 0.00 | 2.11* | −0.50 | |
3-Phosphoglycerate | 0.34 ± 0.01 | 0.15 ± 0.00 | 0.19 ± 0.00 | −1.14* | −0.85 | 0.03 ± 0.00 | 0.07 ± 0.00 | 0.02 ± 0.00 | 1.03* | −1.05* | |
Phosphoenolpyruvate | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.02 ± 0.00 | 0.20 | −0.34 | 0.01 ± 0.00 | 0.05 ± .0.00 | 0.01 ± .0.00 | 2.06* | −0.46 | |
Pyruvate | 0.37 ± 0.01 | 0.53 ± 0.03 | 0.66 ± 0.07 | 0.53 | 0.85 | 5.47 ± 0.35 | 4.35 ± 0.12 | 0.51 ± 0.04 | −0.33 | −3.42* | |
Shikimic path way | Shikimic acid | 3.77 ± 0.15 | 1.20 ± 0.07 | 2.76 ± 0.31 | −1.65* | −0.45 | 3.53 ± 0.24 | 4.81 ± 0.42 | 1.32 ± 0.09 | 0.44 | −1.42* |
Quinic acid | 7.11 ± 0.53 | 1.14 ± 0.04 | 4.36 ± 0.18 | −2.64* | −0.71 | 4.41 ± 0.32 | 4.52 ± 0.13 | 1.55 ± 0.05 | 0.04 | −1.51* | |
Chlorogenic acid | 0.25 ± 0.03 | 0.01 ± 0.00 | 0.20 ± 0.00 | −4.49* | −0.35 | 3.02 ± 0.21 | 5.93 ± 0.56 | 5.70 ± 0.28 | 0.97 | 0.92 | |
Cinnamic acid | 0.86 ± 0.06 | 0.54 ± 0.01 | 14.00 ± 0.65 | −0.67 | 4.03* | 2.68 ± 0.31 | 4.32 ± 0.12 | 10.04 ± 0.59 | 0.69 | 1.90* | |
Ferulic acid | 0.14 ± 0.00 | 0.04 ± 0.00 | 0.34 ± 0.01 | −1.88* | 1.24* | 0.22 ± 0.01 | 0.41 ± 0.02 | 0.27 ± 0.01 | 0.89 | 0.31 | |
Amino acids | Glutamate | 55.50 ± 4.98 | 21.19 ± 0.99 | 92.51 ± 6.35 | −1.39* | 0.74 | 10.55 ± 0.27 | 2.04 ± 0.23 | 3.97 ± 0.31 | −2.37* | −1.41* |
Alanine | 53.01 ± 3.56 | 4.73 ± 0.32 | 64.02 ± 4.01 | −3.49* | 0.27 | 33.98 ± 1.99 | 70.78 ± 5.39 | 7.29 ± 0.51 | 1.06* | −2.22* | |
γ-Aminobutyric acid | 2.77 ± 0.15 | 3.36 ± 0.27 | 2.65 ± 0.19 | 0.28 | −0.06 | 0.41 ± 0.02 | 1.26 ± 0.73 | 0.25 ± 0.03 | 1.61* | −0.74 | |
Valine | 11.35 ± 0.75 | 2.70 ± 0.31 | 11.70 ± 0.85 | −2.07* | 0.04 | 0.74 ± 0.03 | 1.26 ± 0.07 | 0.85 ± 0.02 | 0.78 | 0.21 | |
Serine | 18.16 ± 1.07 | 6.67 ± 0.35 | 15.40 ± 0.87 | −1.44* | −0.24 | 2.61 ± 0.31 | 11.42 ± 0.75 | 3.70 ± 0.18 | 2.13* | 0.50 | |
Aspartate | 4.20 ± 0.12 | 2.34 ± 0.17 | 3.20 ± 0.20 | −0.84* | −0.39 | 1.09 ± 0.04 | 0.56 ± 0.03 | 0.39 ± 0.00 | −1.46* | −0.51 | |
Asparagine | 11.14 ± 0.61 | 14.11 ± 0.57 | 11.65 ± 0.77 | 0.34 | 0.06 | 0.01 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 | 1.04* | −0.84 | |
Threonine | 6.24 ± 0.12 | 2.14 ± 0.10 | 5.42 ± 0.36 | −1.54* | −0.20 | 0.76 ± 0.05 | 1.64 ± 0.19 | 0.39 ± 0.03 | 1.10* | −0.99* | |
Proline | 0.60 ± 0.08 | 8.38 ± 0.69 | 1.58 ± 0.09 | 3.80* | 1.40* | 0.33 ± 0.03 | 19.21 ± 1.14 | 0.82 ± 0.06 | 5.86* | 1.32* | |
Isoleucine | 5.66 ± 0.35 | 2.52 ± 0.16 | 5.21 ± 0.31 | −1.17* | −0.12 | 0.16 ± 0.00 | 0.35 ± 0.03 | 0.22 ± 0.02 | 1.08* | 0.46 | |
Glycine | 8.97 ± 0.59 | 4.09 ± 0.37 | 5.00 ± 0.29 | −1.13* | −0.84 | 4.77 ± 0.25 | 6.33 ± 0.65 | 6.00 ± 0.54 | 0.41 | 0.33 | |
Leucine | 7.06 ± 0.53 | 1.59 ± 0.10 | 4.82 ± 0.21 | −2.15* | −0.55 | 0.24 ± 0.06 | 0.15 ± 0.00 | 0.36 ± 0.03 | −0.68 | 0.62 | |
Glutamine | 0.43 ± 0.15 | 0.01 ± 0.00 | 0.33 ± 0.02 | −5.68* | −0.41 | 0.05 ± 0.00 | 0.03 ± 0.00 | 0.04 ± 0.00 | −0.56 | −0.41 | |
Methionine | 0.98 ± 0.05 | 0.37 ± 0.01 | 0.74 ± 0.02 | −1.39* | −0.41 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.01 ± 0.00 | −0.04 | −1.30* | |
Phenylalanine | 0.35 ± 0.02 | 0.07 ± 0.00 | 0.48 ± 0.04 | −2.25* | 0.47 | 0.02 ± 0.00 | 0.08 ± 0.00 | 0.04 ± 0.00 | 2.04* | 0.84 | |
Sugars | Fructose | 104.07 ± 8.35 | 176.79 ± 7.69 | 118.77 ± 6.51 | 0.76* | 0.19 | 33.13 ± 2.87 | 48.83 ± 2.13 | 36.26 ± 2.00 | 0.56 | 0.13 |
Sucrose | 7.29 ± 0.64 | 22.18 ± 2.09 | 1.34 ± 0.07 | 1.60* | −2.45* | 0.12 ± 0.00 | 0.12 ± 0.00 | 0.12 ± 0.01 | −0.05 | −0.07 | |
Talose | 0.92 ± 0.06 | 2.03 ± 0.10 | 0.47 ± 0.03 | 1.14* | −0.97 | 26.62 ± 2.09 | 40.49 ± 1.57 | 29.32 ± 1.64 | 0.61 | 0.14 | |
Raffinose | 0.49 ± 0.04 | 0.76 ± 0.03 | 0.23 ± 0.01 | 0.64 | −1.11* | 2.81 ± 0.19 | 7.34 ± 0.67 | 3.90 ± 0.25 | 1.38* | 0.47 | |
myo-Inositol | 5.48 ± 0.47 | 13.22 ± 0.96 | 9.59 ± 0.59 | 1.27* | 0.81 | 27.91 ± 2.13 | 41.22 ± 3.61 | 15.01 ± 0.57 | 0.56 | −0.89 | |
Ribose | 2.11 ± 0.15 | 2.84 ± 0.20 | 2.53 ± 0.11 | 0.43 | 0.27 | 3.35 ± 0.17 | 5.07 ± 0.35 | 1.11 ± 0.04 | 0.60 | −1.60* | |
Galactinol | 3.05 ± 0.19 | 3.30 ± 0.56 | 1.48 ± 0.10 | 0.11 | −1.05* | 3.07 ± 0.13 | 10.59 ± 0.63 | 5.49 ± 0.45 | 1.79* | 0.84 | |
Fatty acids | Palmitic acid | 4.65 ± 0.32 | 5.62 ± 0.71 | 6.65 ± 0.43 | 0.27 | 0.52 | 18.69 ± 1.03 | 8.83 ± 0.51 | 34.71 ± 2.17 | −1.08* | 0.89* |
Stearic acid | 1.35 ± 0.07 | 1.24 ± 0.07 | 1.68 ± 0.08 | −0.13 | 0.31 | 2.28 ± 0.14 | 4.12 ± 0.34 | 1.95 ± 0.10 | 0.85 | −0.23 | |
Arachidic acid | 0.15 ± 0.00 | 0.11 ± 0.00 | 0.17 ± 0.00 | −0.43 | 0.22 | 0.38 ± 0.01 | 0.30 ± 0.01 | 0.41 ± 0.02 | −0.34 | 0.12 | |
Linoleic acid | 0.28 ± 0.03 | 0.17 ± 0.00 | 0.32 ± 0.02 | −0.75 | 0.19 | 0.13 ± 0.00 | 0.07 ± 0.00 | 0.26 ± 0.01 | −0.82 | 1.01* | |
Oleic acid | 0.22 ± 0.02 | 0.15 ± 0.00 | 0.23 ± 0.00 | −0.59 | 0.07 | 0.04 ± 0.00 | 0.02 ± 0.00 | 0.09 ± 0.00 | −1.10* | 1.18* |