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Table 1 Subgenome position, conserved amino acid residues (NPA motif, Ar/R filter, Froger's position), the prediction of transmembrane and MIP domains and subcellular localization of B. rapa Aquaporins

From: Genome-wide expression profiling of aquaporin genes confer responses to abiotic and biotic stresses in Brassica rapa

Gene name Sub genome NPA motif Ar/R selectivity filter Froger’s Position (P1 - P5) TMH + MIP Subcellular localization
LB LE H2 H5 LE1 LE2 P1 P2 P3 P4 P5
BrSIP1;1a LF NPT NPA I T P I I A A Y W 6 + 1 CM
BrSIP1;1b MF1 NPT NPA I T P I I A A Y W 6 + 1 CM
BrSIP1;2 LF NPC NPA V T P I I A A Y W 6 + 1 CM
BrSIP2;1a MF1 NPL NPA S K G A F V A Y W 6 + 1 CM
BrSIP2;1b MF2 NPL NPA S K G A F V A Y W 6 + 1 CM
BrSIP2;1c LF NPV NPA S K G A F V A Y W 6 + 1 CM
BrNIP1;2a LF NPA NPG W V A R F S A Y I 6 + 1 CM
BrNIP1;2b MF1 NPA NPG W V A R F S A Y I 6 + 1 CM
BrNIP2;1a LF NPA NPA W V A R F S A Y I 6 + 1 CM
BrNIP2;1b LF NPA NPA W V A R F S A Y I 6 + 1 CM
BrNIP3;1a MF1 NPA NPA W I A R F S A Y I 6 + 1 CM
BrNIP3;1b MF2 NPA NPA W I A R F S A Y I 6 + 1 CM
BrNIP4;1 MF2 NPA NPA W V A R F S A Y I 6 + 1 CM
BrNIP4;2a LF NPA NPA W V A R F S A Y I 6 + 1 CM
BrNIP4;2b MF2 NPA NPA - V A R F S A Y I 4 + 1 CM
BrNIP4;2c MF2 NPA NPA - - A R F S A Y I 3 + 1 CM
BrNIP5;1a MF2 NPS NPV A I G R F T A Y L 6 + 1 CM
BrNIP5;1b MF1 NPS NPV A I A R F T A Y L 6 + 1 CM
BrNIP6;1a MF1 NPA NPV A I A R F T A Y L 6 + 1 CM
BrNIP6;1b LF NPA NPV A I A R F T A Y L 6 + 1 CM
BrNIP7;1 LF NPS NPA A V G R Y S A Y M 6 + 1 CM
BrTIP1;1 MF1 NPA NPA H I A V T A A Y W 6 + 1 V
BrTIP1;2a LF NPA NPA H I A V T A A Y W 6 + 1 V
BrTIP1;2b MF1 NPA NPA H I A V T A A Y W 6 + 1 V
BrTIP1;3 LF NPA NPA H I A V T S A Y W 6 + 1 V
BrTIP2;1a LF NPA NPA H I G R T S A Y W 6 + 1 V
BrTIP2;1b MF2 NPA NPA H I G R T S A Y W 6 + 1 V
BrTIP2;1c MF1 NPA NPA H I G R T S A Y W 5 + 1 V
BrTIP2;2 LF NPA NPA H I G R T S A Y W 6 + 1 V
BrTIP2;3a LF NPA NPA H I G R T S A Y W 6 + 1 V
BrTIP2;3b MF1 NPA NPA H I G R T S A Y W 5 + 1 V
BrTIP3;1a MF1 NPA NPA H I A R T A A Y W 6 + 1 V
BrTIP3;1b LF NPA NPA H I A R T A A Y W 6 + 1 V
BrTIP3;2a LF NPA NPA H M A R T A S Y W 6 + 1 V
BrTIP3;2b MF1 NPA NPA H M A R T A S Y W 6 + 1 V
BrTIP4;1 MF1 NPA NPA H I A R T S A Y W 6 + 1 V
BrTIP5;1 LF NPA NPA N V G C V A A Y W 6 + 1 V and CM
BrPIP1;1a LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;1b MF1 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;2a MF1 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;2b LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;3a MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;3b LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;4 MF1 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP1;5 MF1 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;1 LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;2a MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;2b LF NPA - F - - - Q - - F W 5 + 1 CM
BrPIP2;3a MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;3b LF NPA NPA F H T R Q S A F W 5 + 1 CM
BrPIP2;4a MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;4b MF1 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;4c LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;5a LF NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;5b MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;6 MF2 NPA NPA F H T R Q S A F W 6 + 1 CM
BrPIP2;7a MF2 NPA NPA F H T R M S A F W 6 + 1 CM
BrPIP2;7b LF NPA NPA F H T R M S A F W 6 + 1 CM
BrPIP2;7c MF1 NPA NPA F H T R M S A F W 6 + 1 CM
  1. Blue colour letters denote unusual amino acids in NPA motifs. CM Cell membrane, VVacuole
  2. LF Less Fractioned subgenome, MFs (MF1 and MF2) More Fractioned subgenomes, LB Loop B, LE Loop E, {two half helices (LB and LE)}, NPA Asparagine, Proline, Alanine, AQP contain 6 TM helices (H1 to H6), H2 Helice 2, H5 Helice 5, LE1Loop E1, LE2 Loop E2, Ar/R Aromatic/Arginine, TMH Transmembrane helice