Fig. 5

Molecular organization and polymorphisms of 5S rDNA repeats of Atropa belladonna. a Electrophoretic separation of 5S rDNA PCR products obtained for Acc. Nos 986 and 987; PCR amplification was performed (1) at standard conditions (see Methods), (2) at soft conditions, or (3) at soft conditions using increased (4 μM) concentration of primers; M, molecular weight marker; a ten-fold excess of the PCR product was loaded on gel in the right panel in comparison to the left panel. b Sequence comparison of 5S rDNA variants; Sequences of primers used for PCR amplification are marked by arrows, coding regions are shown as boxes and elements of presumptive external promoter are printed in bold underlined text; Numbers 1 and 2 shown in brackets are referred to Acc. Nos 986 and 987, respectively