Fig. 5
From: MicroRNA 157-targeted SPL genes regulate floral organ size and ovule production in cotton
RNA sequencing analysis of differentially expressed genes between Control and 35S::GhmiR157 transgenic line. a Venn diagram of co-expressed genes among samples. b Analysis of the correlation between samples. c Detection of differentially expressed genes between Control and 35S::GhmiR157 transgenic line based on the NOISeq method. Yellow plots indicate up-regulated genes in Control, blue plots indicate down-regulated genes in Control, brown plots indicate no changed genes. d-m Real-time PCR analysis of differentially expressed genes in floral buds. R.E.L., the relative expression levels calculated using HISTONE3 (AF024716) as a control. The error bars indicate the standard deviation of four biological replicates. Different letters in the plots indicate statistically significant differences at P < 0.05 based on analysis of variance (ANOVA) (Tukey’s multiple comparison test). n GhmiR157 targets were identified using RLM-RACE. Black arrows indicate the position of the target cleavage sites. The numbers next to the black arrows indicate the cleavage frequency. Control, nontransgenic plant segregated from 35S::GhmiR157 transgenic lines in cotton. OV12 and OV38, 35S::GhmiR157 transgenic lines in cotton