Fig. 1
From: MicroRNA 157-targeted SPL genes regulate floral organ size and ovule production in cotton
Over-expressing the GhmiR157 precursor leads to smaller flowers and fewer seeds. a Northern blot analysis of mature miR157 expression in floral buds.5S RNA was used as the RNA loading control. b Images of opened flowers and 30 DPA bolls. c and d The number of ovules per ovary and seed numbers per boll. Different letters indicate statistically significant differences at P < 0.05 based on analysis of variance (ANOVA) (Tukey’s multiple comparison test). The error bars indicate the standard deviation of at least 20 biological replicates. e and f Images of ovaries after removing the valves and mature bolls. OV12, 38, 33, 37 and 35, 35S::GhmiR157 transgenic lines. Control, nontransgenic plant segregated from 35S::GhmiR157 transgenic lines. WT, wild type (Gossypium hirsutum cv. YZ1)