EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in rice

Table 3 Biochemical characterization of germplasm accessions with functional variants discovered through EcoTILLING by sequencing

S. No.	Accessions	Allelic combinations				Mean ± SE
		Allelic combinations				Total starch (%)	AC (%)	RS (%)	HI (%)
		GBSSI	SSI	SS IIa	SS IIIa	Total starch (%)	AC (%)	RS (%)	HI (%)
1.	Os-076	-	2	-	-	82.6 ± 0.3^a	26.0 ± 0.4^b	5.4 ± 0.3^bc	40.8 ± 0.5^ab
2.	Os-468	-	2	2	1	70.7 ± 0.5^h	22.8 ± 0.4^f	7.6 ± 0.4^a	37.8 ± 0.6^b
3.	Os-578	-	1	-	1	79.3 ± 0.4^de	24.5 ± 0.5^c	6.8 ± 0.4^a	42.3 ± 0.5^ab
4.	Os-631	-	1	-	-	74.5 ± 0.5^fg	25.8 ± 0.4^b	6.1 ± 0.2^b	45.6 ± 1.4^ab
5.	Os-678	-	2	-	-	75.2 ± 0.3^f	27.2 ± 0.1^a	5.3 ± 0.2^bc	43.9 ± 0.6^ab
6.	Os-351	-	-	1	-	73.5 ± 0.5^g	23.5 ± 0.3^ef	4.5 ± 0.3^cd	47.7 ± 0.4^ab
7.	Os-211	-	-	2	-	82.1 ± 0.5^ab	23.5 ± 0.3^ef	6.0 ± 0.5^b	47.1 ± 0.6^ab
8.	Os-363	-	-	3	-	80.9 ± 0.5^bc	24.0 ± 0.2^cde	4.1 ± 0.2^d	47.3 ± 0.7^ab
9.	Os-495	-	-	3	1	82.0 ± 0.4^ab	23.7 ± 0.2^cdef	6.9 ± 0.5^b	42.5 ± 0.4^ab
10.	RSM 271	-	-	4	1, 2	82.0 ± 0.4^ab	23.3 ± 0.2^def	7.4 ± 0.4^a	46.5 ± 0.8^ab
11.	RSM 311	-	1	5	1, 2, 3	80.0 ± 0.2^cd	24.2 ± 0.2^cd	7.5 ± 0.3^a	36.3 ± 0.5^b
12.	Pooja	-	-	-	-	78.3 ± 0.5^e	23.2 ± 0.3^ef	2.5 ± 0.3^e	58.2 ± 0.3^a

Different superscripts in the same column denote a statistically significant difference (p ≤ 0.05) for each accession

ISSN: 1471-2229