Table 4 Chloroplast Taraxacum kok-saghyz (TK) and T. officinale (TO) species-specific and intraspecific markers
NO.a | Annotation | Forward primer | Reverse primer | Length | Ta | Enzyme | TK | TO |
|---|---|---|---|---|---|---|---|---|
C1 | Inter space between rbcL and accD | 5′-ACTCTTTCCACCCATCCTGT-3′ | 5′-TGAACCACCATCTTTTCATAGAG-3′ | 287 | 54 | TaqI | Fixed | Fixed |
C2 | accD | 5′-ACTCTTTCCACCCATCCTGT-3′ | 5′-CGCGATCGGGGTTCTTACTA-3′ | 671 | 54 | NcoI | Fixed | Fixed |
C3 | rbcL | 5′-ACCGTTTCTTATTTTGTGCCGA-3′ | ACCCTCAGTAGCAAGATCGC | 677 | 54 | KpnI | Fixed | Fixed |
C4 | rpoC2 | Inner 5′-GAGCACAACCAATCTCTATTCGACCT-3′ | Inner 5′-TCCAAGATGTACTCCTACAAGTAAAGTGG-3′ | TO:215/411 | 58 |  | Fixed | Fixed |
Outer 5′-TATTTCTGTAAGTCCTCGAAATGGAATG-3′ | Outer 5′-AATTTTATTTTTCCATTAGAAGGGGCTC-3′ | TK:251/411 |  | |||||
C5 | Inter space between trnN-GUU and rps15 | 5′-TCAAAGGATCTATGCGCAATCA-3′ | 5′-TCGAGAATTGAAGACCCCTAGT-3′ | 462 | 54 | TaqI | Fixed | Polymorphic |