Fig. 6

Water stress performance of pot-grown Atpub46-48 mutant plants. a Location of the T-DNA insertion in the studied mutants. Exons and introns are shown as wide and narrow lines, respectively. Arrows mark gene orientation. The location of the T-DNA insertions in the mutant lines used in this study are marked by arrows. b Upper panels: analysis of the indicated AtPUB46-48 genes in 1-week old wild type (WT) and the indicated mutants using gene-specific primers that anneal to sequences on both sides of the T-DNA insertions. Lower panels: the expression of ACTIN2 was used as an internal control. c, d Water stress performance of Atpub46-48 mutant plants. Plants were grown in pots for 3 (d) or 4 (c) weeks and then water was withheld from drought treated plants. c, Plants were photographed after 10 days. d Photosynthetic efficiency was assayed 20 days after water withheld. e Detached rosettes of 1 month old pot grown plants were assayed for water loss. Data shown are average ± SE. Statistically significant changes from WT plants (P < 0.05) are marked with asterisks