Fig. 5From: The Arabidopsis paralogs, PUB46 and PUB48, encoding U-box E3 ubiquitin ligases, are essential for plant response to drought stressAtPUB46-48 possesses E3 activity. Self-ubiquitylation of each E3 was assayed in vitro using purified recombinant proteins. Uniquitylated protein (marked by } sign) were detected by western blot using anti-ubiquitin antiserum. a AtPUB46; b AtPUB47; c AtPUB48. a & b had the E2, AtPUBC10 (At5G53300); c had AtUBC8 (At5G41700)Back to article page