Fig. 3

Expression levels of the AtPUB46-48 genes in roots and shoots of 10 d old seedlings untreated or treated with plant hormones. RNA was extracted from roots and shoots, cDNA was prepared and transcripts levels of the indicated genes were analyzed by RT-qPCR. a Root (gray) and shoot (green); transcript levels of all genes were normalized to that of AtPUB46 in the roots. b, c of seedlings were incubated for 6 h with 0.5 x MS without supplements (yellow), or supplemented with 10 μM each of IAA (red), zeatin (blue) or ABA (green). Transcript levels were assayed in roots (b) and shoots (c). Data shown are average ± SE. Expression levels of each gene in the respective organ of non-treated plants were defined as 1