Fig. 9

Trimers, long-OG mix (DP > 8) and flg22 induce MAPK3and MAPK6 phosphorylation. Seedlings were treated for 10 min with trimers (200 μM & 1 mM), long-OG mix (200 μM & 1 mM), flg22 (1 μM), or mock solution (1/2 MS). Each biological replicate is a pool of three wells of seedlings, each containing approximately ten plants. Thirty micrograms of each protein sample was loaded and phosphorylation levels were assessed by immunoblotting using a phospho-p44/42 specific antibody (top). MPK3, MPK4 and MPK6 total protein amounts were assessed using specific antibodies (bottom). The experiment was repeated with a second biological replicate, yielding identical results