Fig. 4

Arabidopsis ferritin-lacking mutants displaying a heat stress-sensitive phenotype were rescued by overexpression of TaFER-5B. a 6-day-old seedlings of WT, fer1-3-4 and fer1-2-3-4 were treated at 45 °C for 2 h, and photographs were taken after 7-d recovery at 22 °C. b 6-day-old seedlings of WT, fer1-2-3-4 and A-CL1 (complemented line) were treated at 45 °C for 2 h, and photographs were taken after 7-d recovery at 22 °C. c Ion leakage assays of fer1-2-3-4, fer1-3-4, WT, overexpression lines A-L1 and A-L2 and complemented line A-CL1 seedlings after heat treatment. d Maximum efficiency of PSII photochemistry (Fv/Fm ratio) of fer1-2-3-4, fer1-3-4, WT, overexpression lines A-L1 and A-L2 and complemented line A-CL1 seedlings at 22 °C and 38 °C. The data represent mean values ± SD of three independent experiments. (* indicates significance at P < 0.05; ** indicates significance at P < 0.01)