Fig. 5

Immunolabelling of lateral roots upon emergence – pectic and AGP epitopes. a Localisation of LM19 in the cells adjacent to the future vascular tissue and in the outer periclinal walls of rhizodermis (arrows), weak signal in other tissues (open arrow). b LM19 at the border of root cap and other cells (arrow). c Punctate signal of LM20 (arrows). d Low (arrow) or no occurrence (arrowhead) of the LM20 epitope in LR (dotted line) and presence in the ground tissue (open arrow). e Occurrence of JIM8 in the AR cells (arrow) and ground tissue of an LR (open arrows, arrowhead). f Magnification of e (asterisk), tonoplast localisation of JIM8 in the ground tissue (open arrow) and some epidermal cells (ellipse). In AR, occurrence in the cortex walls and/or plasmalemma (arrows) and in the extracellular layer (asterisk). f’. Bright field of f, vacuolisation degrees of epidermal (ellipse), meristematic and ground tissue cells (separated by the dotted line). g the JIM13 epitope in the AR cells (arrow) and ground tissue of the LR; wall distribution in differentiated cells (arrowheads) and cytoplasmic localisation in meristematic cells (open arrows). h Magnification of g (asterisk), JIM13 in cytoplasm (arrow), tonoplast (open arrows), plasmalemma and/or cell wall (arrowheads), abundant occurrence in remnants of collapsed cells (asterisks). h’. Bright field of h. i the JIM16 epitope in AR (arrows) and LR cells with an abundant cytoplasmic distribution on the periphery of a primordium (open arrows) in contrast to wall localisation in the ground tissue (arrowheads). j Magnification of i (asterisk), continuous (open arrow) or punctate (arrowheads) tonoplast localisation of JIM16. In the LR epidermis, epitope detected in the plasmalemma, cytoplasm (arrows) and in the extracellular layer (asterisk); in AR punctate distribution in plasmalemma and/or wall (ellipse). j’. Bright field of j. Scale: 100 μm (a, d, e, g, i), 10 μm (b, c, f, f’, h, h’, j, j’)